Contact an Account ManagerStandard SPE Procedure for CHROMABOND® PS-OH- (strong anion exchanger on PS/DVB)
Summary
Type: SPE
Application #: 304035
Detection:
Conditions: Column conditioning: 3 mL methanol, then 3 mL water.
(Do not let run the column dry.)
Sample aspiration: The prepared sample is passed through the column by vacuum or pressure (max. 1000 mL sample volume)
Washing 1: With 3 mL 5 % NH3 in water acidic impurities are removed.
Washing 2 (Elution 1): With 3 mL methanol / water (20:80, v/v) neutral and basic compounds can be removed (eluated).
Elution 2: Compounds are eluted with 2 x 2 mL methanol/acetone (1:1, v/v) + 5 % AcOH or with 2 x 2 mL methanol/acetone (1:1, v/v) + 20 % NH3
Authors: SPE department
Source: Macherey-Nagel
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